工业微生物2011，Vol.41Issue(3)：38-43,6.DOI:10.3969/j.issn.1001-6678.2011.03.008
原生质体激光诱变选育少根根霉脂肪酶高产菌株
Breeding of Rhizopus arrhizus lipase producing strain by laser induced protoplast mutagenesis
摘要
Abstract
The condirions of prootop last forn ation , regeneration and laser mutagenesis of Rhizopus arrhizus BUCT-11 strain were investegated . A s a result, the optinum conditions for protoplast preparation and regeneration of Rhizopus arrhizus BU C T-11 strain were as follow s: 24 h of m ycelium growth time , compound enzyme containing 27 mg/mL of snailase and 53 m g/mL of fibrin enzyme. 1.5 h hydrolysis, hydrolysis tem peralure at 30℃, applying 0.6 mol/ L NH4Cl, 0.02 mol/ L MgSO4 . 7H2O , 0.4 mol/ L CaCl2 phosphate buffer for osm otic stabilizer . The He-Ne laser was inadiated on protoplasts of strain BUCT-11 . The high-yielding lipase mutant num bered Y 007 with good genetic stability was obtained , and its lipase yield in shaking flask scale was up to 151 U/mL , which was 41％ higher than that of the parent strain .关键词
脂肪酶/少根根霉/激光/原生质体/诱变选育Key words
lipase / Rhizopus arrhlius/ laser/ protopjast/ mutaton breeding引用本文复制引用
冀颐之,李政,谭天伟,杜连祥,林强..原生质体激光诱变选育少根根霉脂肪酶高产菌株[J].工业微生物,2011,41(3):38-43,6.基金项目
国家"863"项目(No.2002AA514030) （No.2002AA514030）
国家"十五"攻关项目(No.2001BA708B02208) （No.2001BA708B02208）
国家自然科学基金项目(No.2107600C) （No.2107600C）
中石化项目(No.202059). （No.202059）
