农业环境科学学报2011，Vol.30Issue(5)：946-951,6.
乙草胺降解菌Y-4的分离鉴定及降解特性研究
Isolation and Characterization of a Acetochlor-degrading Strain Y-4 and Its Degrading Characteristics
摘要
Abstract
Acetochlor were widely used to weeding in agriculture and resulted in severely environmental pollution. Bioremediation is an effective and economic method to treat the environment that has been polluted by acetochlor. So researchers paid much attention in this area. In the present study, a bacterial strain Y-4 capable of utilizing acetochlor as sole carbon and energy sources was isolated from the sludge that have long been subjected to acetochlor pollution by enrichment method The Y-4 cells were short rod, Gram nesative. The colonies on LB media were light yellow in color, circular, and smooth. Strain Y-4 was identified preliminarily as Shinella sp. based on its physiological and biochemical characters and the result of the 16S rDNA homologue sequence analysis. The Y-4 strain could effectively degrade acetochlor ranging of 5-200 mg·L-1. 50 mg· L-1 acetochlor could be degraded at a rate of 83.3％ by Y-4 strain in 48 h. The optimal initial pH value and temperature for acetochlor degradation by Y-4 was 8.0 and 30 ℃, respectively. Adding extra glucose or nitrogen source promoted the growth of strain Y-4, but influence the degradation of acetochlor slightly. The ion Mg2+ could promote the strain to degrade acetochlor slightly, and Ni2+, Co2+, Hg2+ could inhibit it when it was at low level. However, Zn2+, Fe2+ had no effect on the degradation of acetochlor. Strain Y-4 could also degrade some other pesticides such as pretilachlor and butachlor.关键词
乙草胺/降解/申氏杆菌Key words
acetochlor/ degradation/ Shinella sp.分类
资源环境引用本文复制引用
倪俊,沈维亮,闫新,李顺鹏..乙草胺降解菌Y-4的分离鉴定及降解特性研究[J].农业环境科学学报,2011,30(5):946-951,6.基金项目
江苏省科技支撑计划(BE2009670) （BE2009670）
