陕西医学杂志2011，Vol.40Issue(1)：7-10,4.
异氟醚对未成熟兔缺血再灌注心肌细胞间黏附分子-1、核因子-κB表达的影响
Effects of isoflurane preconditioning on myocardial cells and the expression of ICAM-1 and NF-κB after ischemia reperfusion in immature rabbits
摘要
Abstract
Objective:To investigate the effects of isoflurane preconditioning on apoptosis of immature myocardial cells and the expression of ICAM-1 and NF-KB after ischemia reperfusion in immature rabbits. To discuss the protective mechanism of isoflurane against ischemia-reperfusion injury of immature myocardial cell. Methods: 32 Japanese Big-ear rabbits were randomly divided into four groups: The sham operation group,the heart were exposed ,but the coronary artery were not ligatured;The ischemia-reperfusion group:the left anterior descending coronary artery were ligatured 30 min, then followed by 2 hour reperfusion;The isoflurane preconditioned group : the rabbits were treated with isoflurane(1. 1 ％) 30 min before ischemia. In the gliben clamide group: the rabbits were treated with gliben clamide (O. 5mg/kg) before breathing in isoflurane. Apoptosis of myocardial cells were detected by TUNEL method,the expression of ICAM-1 and NF-KB were examined by immunohistochemical technique,and the fine cells in isoflurane group and fake operation group were observed under electron microscope as well. Results: The apoptosis of myocardial ischemiareperfusion group and gliben clamide group(P＜0. 05). The expression of ICAM-1 and NF-κB were significantly lower than those in ischemia-reperfusion and gliben clamide group(P＜0. 05). The injury extent of isoflurane group was smaller than that in ischemia-reperfusion and gliben clamide group seen from the changes of the celluar structure under electron microscope. Conclusion: Isoflurane can decrease apoptosis of myocardial cells induced by ischemia-reperfusion,lsoflurane preconditioning may provide obviously myocardial protection against ischemia-reperfusion injury in immature rabbit heart, by decreasing the expression of ICAM-1、NF-κ and K-ATP channels is likely involved in this protective mechanism.关键词
心肌缺血/病理生理学/再灌注损伤/病理生理学/NF-κB/代谢/细胞间黏附分子-1/异氟醚/治疗应用/动物,实验/兔Key words
Myocardial ischemia/physiopathology/ Reperfusion injury/physiopathology /NF-kappa B/metabolism / @ICAM-1 / Isofurane/therapeutic use/ Animals,laboratory/ Rabbits分类
医药卫生引用本文复制引用
陈权,刘国利,李平,张锦英..异氟醚对未成熟兔缺血再灌注心肌细胞间黏附分子-1、核因子-κB表达的影响[J].陕西医学杂志,2011,40(1):7-10,4.基金项目
辽宁省教育厅高等学校科研项目(No 2008400) （No 2008400）
