浙江医学2011，Vol.33Issue(6)：836-839,4.
尼妥珠单抗对鼻咽癌CNE-2细胞放射敏感性的影响
Effect of nimotuzumab on radiosensitivity of nasopharyngeal carcinoma CNE-2 cells
花永虹 1马胜林 2付真富 2胡巧英 2杜灵彬 2江皓1
作者信息
- 1. 310053,杭州,浙江中医药大学研究生院
- 2. 浙江省肿瘤医院放疗科
- 折叠
摘要
Abstract
Objective To investigate radiosensitive effects of nimotuzumab on nasopharyngeal carcinoma cell line CNE- 2.Methods The 50％ inhibition concerntration (IC50) of nimotuzumab on cell proliferation was measured by MTT.Cell survival was determined by clonogenic assay, single- hit multi- target mode was used to plot survival curve.The cell cycle and apoptosis were measured by flow cytometry.Results Nimotuzumab inhibited proliferation CNE- 2 cells, the 24h IC50 was 945μg/ml.The radiosensitization enhancement ratios (SERDO) at 0.2× IC50 and 0.3× IC50 of nimotuzumab were 1.26 and 1.38 respectively, and the nimotuzumab followed by radiation had a higher apoptosis rate and G0 /G1 phase cells (P＜ 0.05).Conclusion Nimotuzumab can enhance the radiosentivity of nasopharyngeal carcinoma cell line CNE- 2, which was possibly associated with inhibiting cell proliferation, inducing apoptosis, and altering cellphase distribution.关键词
鼻咽癌/尼妥珠单抗/放射敏感性Key words
Nasopharyngeal carcinoma / Nimotuzumab / Radiosensitivity引用本文复制引用
花永虹,马胜林,付真富,胡巧英,杜灵彬,江皓..尼妥珠单抗对鼻咽癌CNE-2细胞放射敏感性的影响[J].浙江医学,2011,33(6):836-839,4.
