浙江医学2011，Vol.33Issue(6)：843-846,4.
中效原理在胃癌联合化疗中的应用及药物抗癌机制的初步探索
Application of median-effect principle in combined chemotherapy for gastric cancer and preliminary exploration of three drugs' anti-cancer mechanisms
胡金喜 1林赛锋 1曾呈军 1刘磊 1周磊 1葛小刚 1郑志强1
作者信息
- 1. 325027,温州医学院附属第二医院普外科
- 折叠
摘要
Abstract
Objective To analyze the effects of chemotherapy induced by curcumin combined with cisplatin or celecoxib in the human gastric cancer cell line SGC- 7901 cells by using the combination index method of Chou- Talalay (median- effect principle), and to explore the anti- cancer mechanisms of the three drugs.Methods The experimental groups were divided into six sub- groups: curcumin, cisplatin, celecoxib, combination ofcurcumin with cisplatin, combination ofcurcumin with celecoxib; The control group without any drug treatment.MTT assay was performed to detect the proliferation of SGC- 7901 cells, and the median- effect principle was used to analyze the effects oftheir combinations.Flow cytometry was performed to detect the apoptosis rate.The expression of COX- 2 mRNA was analyzed by fqRT-PCR.The activation ofCaspase- 3 was detected by spectrophotometry.Results Each of curcumin, cisplatin and celecoxib could inhibit the proliferation of SGC-7901 cells in a concentration- dependent manner.The combination ofcurcumin with cisplatin or celecoxib could strengthen the depressive effect.Synergistic effects were detected in these combinations.All of the three drugs could induce apoptos is and the apoptosis rate of the two drugs treated groups was significantly higher than the one drug treated groups (all P ＜0.01).The expression ofCOX- 2 mRNA in cells was lower in the drugs treated groups than in negative control group (all P＜0.01).The activation ofCaspase- 3 in cells was higherin the drugs treated groups than in negative controlgroup (all P ＜0.01).Conclusion Within a certain concentration, each of curcumin, cisplatin and celecoxib could inhibit the proliferation of gastric cancer cells SGC-7901 and induce apoptosis.Synergistic effects were detected in the combination ofcurcumin and cisplatin or celecoxib, perhaps correlated with that all the three drugs could inhibit the expression of COX- 2 mRNA and increase the activation ofCaspase- 3.关键词
中效原理/姜黄素/顺铂/塞来昔布/环氧化酶-2Key words
Median- effect principle/ Curcumin/ Cisplatin/ Celecoxib/ COX- 2引用本文复制引用
胡金喜,林赛锋,曾呈军,刘磊,周磊,葛小刚,郑志强..中效原理在胃癌联合化疗中的应用及药物抗癌机制的初步探索[J].浙江医学,2011,33(6):843-846,4.
