军事医学2011，Vol.35Issue(4)：278-281,4.
1-磷酸鞘氨醇促进大鼠血管平滑肌细胞迁移的实验研究
Migration of rat vascular smooth muscle cells promoted by S1P:an experimental study
摘要
Abstract
Objective To examine the role of sphingosine 1-phosphate ( S1 P) signals in the regulation of rat vascular smooth muscle cells migration, and to offer the new ideas for tumor angiogenesis and cardiovascular disease. Methods Rat vascular smooth muscle cells were isolated and characterized by expression of α-smooth muscle(α-SM) actin. The hypoxia models were induced by 1％ O2 incubators and CoCl2. The expression patterns of S1P receptor mRNAs in rat vascular smooth muscle cells were analyzed using RT-PCR analysis. The Transwells were used for the role of S1P signals in the regulation of rat vascular smooth muscle cells migration, that was confirmed by the S1P receptor antagonist. Results and Conclusion SPK1 and the S1P receptor, rS1P1, rS1 P2 and rS1 P3 were expressed in rat vascular smooth muscle cells. Hypoxia induced by 1％ O2 and CoCl2 could upregulate SPK1 expression and activity of rat vascular smooth muscle cells. S1P promotes the migration of rat vascular smooth muscle cells through S1P receptor subtypes rS1P2.关键词
低氧/1-磷酸鞘氨醇/鞘氨醇激酶/血管平滑肌细胞/细胞迁移Key words
hypoxia/ sphingosine l-phosphate/ sphingosine kinase/ vascular smooth muscle cells/ migration分类
生物科学引用本文复制引用
郭强,张凯,刘谟焓,孔庆龙..1-磷酸鞘氨醇促进大鼠血管平滑肌细胞迁移的实验研究[J].军事医学,2011,35(4):278-281,4.基金项目
国家自然科学基金项目(30901462) （30901462）
