北京中医药大学学报2011，Vol.34Issue(4)：271-273,285,4.
正交试验法优选广痛消中芍药苷的提取工艺
Optimized extraction technique of paeoniflorin in Guangtongxiao Foam Aerosol with orthogonal design test*
摘要
Abstract
Objective To optimize the extraction technique of paeoniflorin in Guangtongxiao Foam Aerosol. Methods Taking the content of paeoniflorin as index, the multiple of extraction solvent ( A), number of extraction (B) and period of extraction (C) were observed by using L9 ( 34 ) orthogonal design test for determining the best extraction technique. The HPLC procedure was performed on the chromatographic column of Agilent Extend (4. 6 mm × 150 mm, 5 μm) at 30℃ and the mobile phase was acetonitrile-0. 1％ phosphoric acid solution in gradient elution program (12:88 ). The flow velocity was 0. 8 mL/min and the detection wavelength was 230 nm. Results The sequence of influence of all factors on paeoniflorin extraction volume was A ＞ B ＞ C and the best extraction technique was A3B2C1 (adding water by 8 times, extracting for 2 times and for 1 hour each time). The extractive weight of paeoniflorin was 449. 502 2 mg, which was the most extraction quantity. Conclusion The technique is reasonable, stable and available, and provides a foundation for the further production and quality control of Guangtongxiao Foam Aerosol.关键词
广痛消泡沫气雾剂/芍药苷/正交设计/提取工艺Key words
Guangtongxiao Foam Aerosol/ paeoniflorin/ orthogonal design/ extraction technique分类
医药卫生引用本文复制引用
彭俊付,刘勇,刘永刚,赵宝明,张书信,胡博,李天罡..正交试验法优选广痛消中芍药苷的提取工艺[J].北京中医药大学学报,2011,34(4):271-273,285,4.基金项目
国家重大新药创制项目(No.2009ZX09502-008-6) （No.2009ZX09502-008-6）
