寄生虫与医学昆虫学报2011，Vol.18Issue(2)：81-83,3.DOI:10.3969/j.issn.1005-0507.2011.02.004
新疆伊犁那拉提草原啮齿动物感染恙虫病东方体调查
Investigation on rodents infection of Orientia tsutsugamushi in some areas of Nalati Grassland, Xinjiang Uygur Auto.Reg., China
摘要
Abstract
DNAs were extracted from spleens of the rodents captured from Nalati area of Xinjiang Uygur Auto. Reg. , and nested-polymerase chain reaction ( nPCR ) was performed to detect the Orientia tsutsugamushi ( Ot )-Sta 56 gene. Some positive samples were sequenced and analyzed by BLAST of NCBI network. A total of 67 rodents and 29 marmots were captured in Nalati area. The positive fragments were detected from the spleens of 3 mice and 2 marmots , including Karp and Saitama genotype. The detection rates of Ot from Mus musculus, Cricetulus migratorius and Marmota barbacina were 6. 06％, 2. 94％ and 6. 90％ , respectively. The homology of one fragment of the amplified gene nucleotide sequence to Karp strain was 99％ . The other fragments could not be sequenced. It was presumed that the Karp strain was the main gene type, and the Saitama strain might exist in this area.关键词
恙虫病东方体/恙虫病/流行病学Key words
Orientia tsutsugamushi/ Scrub typhus/ Epidemiology引用本文复制引用
党荣理,任立松,高金拽,马德新,刘晓明..新疆伊犁那拉提草原啮齿动物感染恙虫病东方体调查[J].寄生虫与医学昆虫学报,2011,18(2):81-83,3.基金项目
总后卫生部"十一五"第二批基金资助项目(08G014) （08G014）
