寄生虫与医学昆虫学报2011，Vol.18Issue(2)：84-89,6.DOI:10.3969/j.issn.1005-0507.2011.02.005
不同地区不同生境按蚊种群的分子鉴定及rDNA-ITS2序列分析
Molecule identification and rDNA-ITS2 sequence analysis of Anopheles from different provinces and habitats
摘要
Abstract
The partial rDNA-ITS2 segments of Anopheles from Jiangsu, Anhui, Hunan, Yunnan, Hainan provinces and Guangxi Zhuang autonomous region were amplified using PCR and then were sequenced. The sequence alignment results of rDNA-ITS2 indicated that all the samples obtained from different provinces and habitats belonged to Anopheles sinensis.There were high identities between the samples group which ranging from 98. 2％ to 100％ . The identities between the samples and An. hycauns ( An. liangshanensis, An. kunmingensis , An. lesteri and An. anrhropophagus , An. nigerrimus An.peditaeniatus ) were 79. 1％ -82％ , 76. 1％ -77. 1％ , 60. 9％ -63. 9％ , respectively. Phylogenetic analysis results showed that all the segments obtained from the fields were clustered together and formed two separated branches with the segments of An. hycauns form GenBank.关键词
按蚊/分子鉴定/rDNA-ITS2/序列分析/同源性Key words
Anopheles/ Molecule Identification/ rDNA-ITS2/ Sequence analysis/ Identity引用本文复制引用
谭伟龙,赵彤言,董言德,汪中明,李春晓..不同地区不同生境按蚊种群的分子鉴定及rDNA-ITS2序列分析[J].寄生虫与医学昆虫学报,2011,18(2):84-89,6.基金项目
国家科技重大专项(重要病媒生物监测与传播病原体检测的关键技术研究,NO:2008ZX10004-010) （重要病媒生物监测与传播病原体检测的关键技术研究,NO:2008ZX10004-010）
