农药学学报2011，Vol.13Issue(2)：149-154,6.DOI:10.3969/j.issn.1008-7303.2011.02.09
杨梅叶抑菌活性成分初步分离与鉴定
Isolation and identification of antifungal constituents from the leaf of Myrica rubra
摘要
Abstract
Methanol extract of the leaves of Myrica rubra was further partitioned with petroleum ether,chloroform, ethyl acetate and n-butanol respectively and obtained four crude extracts. Antifungal activity of the four crude extracts was tested . It showed that ethyl acetate and n-butanol extract had higher activity. Their structures were identified by ESI-MS, 1H NMR and 13C NMR as maslinic acid( 1 ) ,3-O-trans-p-coumaroyl maslinic acid ( 2 ), 3 -O-galloyl-dihydromyricetin ( 3 ), quercetin-7 -O-rhamonside ( 4 ),myricetin-3-O-rhamnoside ( 5 ), myricetin-3-O-rhamnoside ( 3-O-galloyl ) ( 6 ), myricetin-3-O-rhamnoside(2-O-galloyl) (7). The inhibition effects of compound 3,4,5 and 6 had been tested by method of spore germination and the result showed that all the 4 compounds have strong inhibiting effect on the tested plant pathogens. Botrytis cinerea and Setosphaeria turcica which LC50 velues were between 3.44 -4.59 mg/L and 8.85 - 11.6 mg/L,respectively.关键词
杨梅叶/萃取分离/抑菌活性/活性成分Key words
Myrica rubra leaves/ extract and isolation/ antifungal activity/ active component分类
农业科技引用本文复制引用
张绍勇,李桥,陈安良..杨梅叶抑菌活性成分初步分离与鉴定[J].农药学学报,2011,13(2):149-154,6.基金项目
浙江省科技厅面上项目(2008C22G2100038). （2008C22G2100038）
