农业环境科学学报2011，Vol.30Issue(4)：645-649,5.
保护剂减轻氯嘧磺隆对玉米残留药害的作用机理
Mechanism of Herbicide Safener for Reducing the Phytotoxicity of Chlorimuron-ethyl to Maize
摘要
Abstract
Herbicide safener can reduce phytotoxicity of herbicides to crops by influencing absorption and conduction of crops, and inducing the activity increase of GSTs and its target enzyme ALS.There may be two mechanisms of inducing the increase of GSTs activity.One is to induce the original GSTs isoenzymes over-expressed, the other is to induce synthesis of new GSTs isoenzymes.In this paper, physiological and biochemical tests and semi-quantitative PCR method were employed to study the effect of herbicide safener HN on the activity ALS and GSTs of maize as well as the effect of both of chlorimuron-ethyl and safener HN on the GSTs mRNA expression.The results showed that herbicide safener HN had effect of induction on ALS and GSTs of six maize varieties, but the increase percentage on different varieties showed a little difference.For chlorimuron-ethyl sensitive maize variety Dongtian 3, the inducing percentage of GSTs reached 59.18％, while its ALS increased 1.29 times in leaf and 2.30 times in root.For chlorimuron-ethyl tolerant maize variety Tunyu 88, the inducing percentage of GSTs was 19.26％, while its ALS increased 1.07 times in leaf and 2.07 times in root.Both of chlorimuron-ethyl and herbicide safener could induce relative increasing expression of GSTs mRNA and herbicide safener had stronger induction than chlorimuron-ethyl.关键词
保护剂/氯嘧磺隆/玉米/GSTs/mRNA表达量Key words
herbicide safener /chlorimuron-ethyl/ maize/ GSTs/ expression of mRNA分类
资源环境引用本文复制引用
郭玉莲..保护剂减轻氯嘧磺隆对玉米残留药害的作用机理[J].农业环境科学学报,2011,30(4):645-649,5.基金项目
黑龙江省农科院博士后基金项目(LRB08-350) （LRB08-350）
哈尔滨市科技攻关项目(2008AA6CH047) （2008AA6CH047）
