南京医科大学学报（自然科学版）2011，Vol.31Issue(5)：697-701,5.
齐墩果酸预处理对大鼠肝脏缺血再灌注损伤过程中IKK/I-κB/NF-κB通路的影响
lnfluence of oleanolic acid pretreatment to the lKK/l-KB/NF-KB signaling pathway during rat's HlRl
摘要
Abstract
Objective:To investigate the influence of oleanolic acid (OA) pretreatment to rat's IKK/I-KB/NF-KB signaling pathway around its hepatic ischemic/reperfusion injury (HIRI). Methods:One hundred and twenty eight male Sprague-Dawley (SD) rats were randomly divided into sham group(SH),ischemic/reperfusion group(IR),0.5％ sodium carboxymethycellulose(CMC-Na) group(CM) and OA+0.5％ CMC-Na group(OA). Before the operation,the rats of each group received intragastric administration of corresponding solution once a day for seven days (OA group with 100 mg/kg of OA dissolved in 0.5％ CMC-Na,SH group and IR group with water of the same volume,CM group with 0.5％ CMC-Na of the same volume). At the 8th day,rats were suffered from segmental(70％) hepatic ischemia for 60 min and then followed with different periods of reperfusion. The hepatic tissue were obtained at 0 h,3 h,and 6 h after the reperfusion.The expression of IKK2 and I-KBα in the cytoplasm and NF-KB p65 in the nucleus were evaluated by the method of Western blotting. Results:There was no IKK2 expression in the cytoplasm,and only few of NF-KB p65 was detected in the nucleus before operation or Oh after reperfusion in each group. Meanwhile,the volume of I-KBα detected in the cytoplasm has no significant difference among four groups at the same point of time. The expression of IKK2 in the cytoplasm and NF-KB p65 in the nucleus in the SH group was much lower than those in other three groups(P ＜ 0.05) ,while those proteins detected in OA group is less than those in CM group and IR group(P ＜0.05)at 3h or 6 h after reperfusion. At these two points of time,the expression of I-KBα in SH group was higher than that in the other three groups (P ＜ 0.05),and this protein detected in OA group is more than that in CM group and IR group (P ＜ 0.05).The expressions of IKK2 and I-KBα in the cytoplasm and NF-KB p65 in nucleus between CM group and IR group had no significant difference at each point of time (P ＞ 0.05). Conclusion:HIRI would increase the expression of IKK2 in the cytoplasm,leading to the phosphorylation of I-KB,activating NF-KB and making it to transloeate into the nucleus,and then the activated NF-KB which could mediate inflammatory response eventually caused damage of liver cells. The pretreatment of OA can inhibit the activating of the signaling pathway of IKK/I-KB/NF-KB,which may be one of the mechanisms for OA alleviating HIRI.关键词
齐墩果酸/糖原磷酸化酶抑制剂/缺血再灌注损伤/NF-κB/I-κB/IKKKey words
oleanohc acid/ ischemic/reperfusion injury/ NF-KB/ I-KB/ IKK.分类
医药卫生引用本文复制引用
何明枫,华福洲,张杰,许仄平,孙宏斌,钱燕宁..齐墩果酸预处理对大鼠肝脏缺血再灌注损伤过程中IKK/I-κB/NF-κB通路的影响[J].南京医科大学学报（自然科学版）,2011,31(5):697-701,5.基金项目
国家自然科学基金资助(30672523) （30672523）
