南京医科大学学报（自然科学版）2011，Vol.31Issue(5)：702-705,4.
17β-estradiol通过促进PSD95/nNOS耦联抑制皮层海马神经元的存活
17β-estradiol inhibits the survival of cortical hippocampal neuron by promotingthe coupling of nNOS and PSD95
摘要
Abstract
Objective:To detect the effects of 17β-estradiol on the survival of cortical hippocampal neurons and determine whether it is associated with nNOS/PSD95 complexes. Methods: ICR mice was adopted to culture cortical hippocampal neurons. Eight days later, lactate dehydrogenase(LDH) release rate were examined in cultured cortical hippocampal neurons exposed to DMSO or 10 μmol/L 17β-estradiol for 30 min. The neurons were photographed through a light microscope. Meanwhile, nNOS/PSD95 complexes of neurons treated with DMSO, 10 nmol/L 17β-estradiol and 10 μmo1/L 17β-estradiol were measured by co-immunoprecipitation. Results:At 30 minutes after administration, 10 μmol/L 17β-estradiol treatment increased LDH release rate and nNOS/PSD95 conjugating in cortical hippocampal neurons, and 10 nmol/L 17β-estradiol treatment had no influence on nNOS/PSD95 conjugating. Conclusion: 17β-estradiol of 10 μmol/L could injure cortical hippocampal neurons by promoting the conjugating of nNOS and PSD95.关键词
nNOS/PSD95/雌激素/17β-estradiol/细胞死亡Key words
nNOS/PSD95/estrogen/17β-estradiol/cell death分类
医药卫生引用本文复制引用
邬丹莲,胡瑶,朱明媚,朱东亚..17β-estradiol通过促进PSD95/nNOS耦联抑制皮层海马神经元的存活[J].南京医科大学学报（自然科学版）,2011,31(5):702-705,4.基金项目
国家自然科学基金(30971021) （30971021）
