北京林业大学学报2011，Vol.33Issue(3)：21-25,5.
沙冬青GATA型锌指蛋白基因序列及表达分析
Sequence analysis and expression pattern of AmZFPG encoding a GATA type zinc finger protein in Ammopiptanthus mongolicus.
摘要
Abstract
We designed special primers according to the zinc finger protein gene sequence in expressed sequence tags from a cold- and drought-treated Ammopiptanthus mongolicus cDNA library and amplified cDNA of AmZFPG by PCR. The cDNA of AmZFPG is comprised of 669 base pairs which encode a zinc finger protein with 273 amino acids and a molecular weight of 25 kD. Bioinformatic analysis indicates that AmZFPG belongs to GATA-binding protein family and shares higher homology with given zinc finger proteins which are involved in stress resistance in other plant species. Phylogenetic analysis indicates that AmZFPG is most closely related to Medicago sativa proline-rich cell wall protein. Semiquantitative RTPCR analysis shows that the expression of AmZFPG is constitutive and up-regulated under cold and drought stresses and that AmZFPG is related to response to drought and chilling stresses.关键词
沙冬青/锌指蛋白/序列分析/表达分析Key words
Ammopiptanthu mongolicus/ zinc finger protein/ sequence analysis/ expression analysis分类
农业科技引用本文复制引用
史军娜,刘美芹,师静,王艳萍,智冠华,陈玉珍,卢存福..沙冬青GATA型锌指蛋白基因序列及表达分析[J].北京林业大学学报,2011,33(3):21-25,5.基金项目
国家自然科学基金项目(30700559,30671476). （30700559,30671476）
