畜牧兽医学报2011，Vol.42Issue(5)：621-628,8.
莱芜猪PID1基因的功能分析及表达谱研究
Functional Analysis and Tissue Specific Expression Profile of PID1 Gene in Laiwu Pig
摘要
Abstract
In order to understand the structure and function of porcine PID1 gene, the molecular structure and function of porcine PID1 from the Laiwu pig sample were analyzed. The mRNA expression profile of the porcine PID1 gene in ten tissues (heart, longissimus dorsi, spleen, liver, lung, small and large intestine, brain, fat and spinal) were examined by QRT-PCR. Bioinformatic analysis revealed that the porcine PIDI protein was located in the cytoplasmic, encoded 217 amino acids with a molecular weight of 54 ku and a pl of 5. 11. PID1 was a non-transmembrane protein and was an unsecreted and hydrophilic protein, α helix occupied the higher proportion in secondary structure. In addition, PID1 gene had a phosphotyrosine-binding (PTB) domain on the C terminal of protein. Laiwu pig, homo spiens and pan troglodytes were closed in the phylogenetic tree. Real-time PCR analysis showed that the PID1 gene was expressed in various tissues, but PID1 mRNA abundances, on the whole, tended to be heart ＞ brain ＞ liver ＞ fat ＞longissimus dorsi ＞ lung＞ spinal＞ large intestine＞ spleen, small intestine was not detected.The results implied that PID1 may be related to insulin signal pathway and regulate the expression of GLUT4, which showed us its extensive functions for transcription.关键词
莱芜猪/PID1基因/生物信息学/表达谱分类
农业科技引用本文复制引用
钱源,曾勇庆,崔景香,陈其美,宋一萍,杜金芳,陈伟..莱芜猪PID1基因的功能分析及表达谱研究[J].畜牧兽医学报,2011,42(5):621-628,8.基金项目
国家转基因重大专项(2008ZX08006-002) （2008ZX08006-002）
国家高技术研究发展计划"863"重点项目(2008AA101008) （2008AA101008）
山东省农业良种工程重大项目(2009LZ012-03) （2009LZ012-03）
山东省自然科学基金项目(Y2008D32) （Y2008D32）
