畜牧兽医学报2011，Vol.42Issue(5)：635-640,6.
猪卵母细胞线粒体分布及线粒体DNA拷贝数变化
Mitochondrial Distribution and Mitochondrial DNA Copy Number in Porcine Oocvte at Different Periods during in vitro Maturation
摘要
Abstract
To observe mitochondrial distribution and mitochondrial DNA copy number changes in porcine oocytes at different periods during in vitro maturation. Mito-Tracker Green and real-time Quantitative PCR were used to respectively detect the mitochondrial distribution and mitochondrial DNA copy number in porcine oocytes. The results showed that the mitochondrial distribution was gradually translocated from the cortex to the perinuclear area of oocytes, and then spread in the cytoplasm thoroughly, and mitochondrial clusters became larger, stain became deeper. Mitochondrial DNA copy number of 0, 11, 22 h were (2519. 52±940. 39), (3421. 47±345. 71) ,(9747. 58±1928. 24), respectively, and there was no significant difference (P＞0. 05) among them. Mitochondrial DNA copy number of 33 h was (39913. 61±1180. 26), which was significantly higher than those of 0,11,22 h (P＜0. 05). Mitochondrial DNA copy number of 44 h was (130074. 30±78119. 45), which was significantly higher than that of 33 h (P＜0. 05). In conclusion, as the oocyte maturation process forward, mitochondrial activity enhanced and mitochondrial DNA copy number increased.关键词
猪卵母细胞/体外成熟/线粒体分布/线粒体DNA拷贝数分类
农业科技引用本文复制引用
成文敏,霍金龙,信吉阁,潘伟荣,黄言,魏红江,曾养志..猪卵母细胞线粒体分布及线粒体DNA拷贝数变化[J].畜牧兽医学报,2011,42(5):635-640,6.基金项目
国家自然科学基金项目(30800785) （30800785）
云南省教育厅科学研究基金重点项目(0920042) （0920042）
