畜牧兽医学报2011，Vol.42Issue(5)：641-649,9.
DSG4基因的SNPs筛查及其与裘用绵羊羊毛性状关系的研究
Polymorphism of DSG4 Gene and Its Association with Wool Traits in Fur Sheep
摘要
Abstract
By SSCP assay, polymorphisms of DSG4 gene were screened in Tan sheep (n=118)and their crossbred with Small-tailed Han sheep (n=82) in this study. The relationship between polymorphisms of DSG4 gene and length and crimp number of scapular wool plait was analyzed by least square linear model. The results showed 16 SNPs and a TTG indel in sheep DSG4 gene,in which one each SNP located at 5'UTR and 3'UTR, respectively, 11 SNPs on coding regionwith four of them leading to amino acid substitutions, and three SNPs on introns. The TTG indel led to an amino acid substitution from Ser to Iso and also a Gly indel. Interestingly, six variations in coding region and the SNP at 3'UTR were in complete linkage disequilibrium, forming combinated genotypes named as PPRR, PQRS and QQSS. In the crossbreds, individuals with QQSS genotype had a significantly higher least square mean than individuals with genotype PPRR or PQRS on length of scapular wool plaits at birth (P＜0. 01) , crimp number of scapular wool plait at birth (P＜0. 01), crimp number of scapular wool plaits at weaning (P＜0. 01). The individuals with genotype QQSS also had a significantly higher crimp number of rump wool plait at weaning than individuals with genotype PPRR (P＜0. 05). However, as for Tan sheep, the individuals with genotype ooss only had a slightly higher least square mean than individuals with genotype PPRR or PQRS on the above traits (P＞0. 05). These data preliminarily indicate ooss is the most favorable genotype affecting length and crimp number of wool plait. These variations provide opportunities for further investigating the effects of DSG4 on wool traits in sheep.关键词
滩羊/DSG4基因/SNP/羊毛性状分类
农业科技引用本文复制引用
张戈,张元跃,韩建林,关伟军,李颖康,许斌,张秀陶,马月辉..DSG4基因的SNPs筛查及其与裘用绵羊羊毛性状关系的研究[J].畜牧兽医学报,2011,42(5):641-649,9.基金项目
现代农业产业技术体系建设专项资金(nycytx-40-5) （nycytx-40-5）
科技支撑计划(2006BAD13B08 2008BADB2B01) （2006BAD13B08 2008BADB2B01）
转基因生物新品种培育科技重大专项(2008ZX08009-003) （2008ZX08009-003）
