畜牧兽医学报2011，Vol.42Issue(5)：711-716,6.
抗猪甲型H1N1流感病毒血凝素蛋白单克隆抗体的制备与特性分析
Preparation and Characterization of Monoclonal Antibody Against Hemagglutinin Protein of 2009 Strain of H1N1 Swine Influenza Virus
摘要
Abstract
This experiment was conducted to prepare monoclonal antibodies specific to the hemagglutinin protein of H1N1 swine influenza virus (SIV) , A/Swine/Heilongjiang/44/2009. Hemagglutinin (HA) gene of the SIV was amplified by RT-PCR, and then the HA gene was subcloned into the eukaryotic express vector pCAGGS. The recombinant plasmid was named as pCAGGSHA. Expression of HA protein in 293T cell was confirmed by indirect immunofluorescence assay (IFA). Five weeks old BALB/c mice were immunized with 100 μg pCAGGS-HA plasmid each time, and 4 hybridoma cell lines secreting MAbs against the HA protein were obtained, which were designated as 11G7, 3C10, 3G3 and 2B11, respectively. The mice ascite of 11G7 has a HItiter of 14log2, and a neutralization titer of 1:8 192. Furthermore, 11G7 could only react with the 2009 H1N1 SIV, and not with other H1N1, H1N2, H3N2, H5N1 and H9N2 subtype SIVs,which provide an useful tool for differential diagnosis between 2009 H1N1 and other traditional subtype SIVs.关键词
甲型H1N1猪流感病毒/HA/MAb分类
农业科技引用本文复制引用
张健,陈艳,乔传玲,杨焕良,唐续,辛晓光,陈化兰..抗猪甲型H1N1流感病毒血凝素蛋白单克隆抗体的制备与特性分析[J].畜牧兽医学报,2011,42(5):711-716,6.基金项目
哈尔滨市科技攻关计划项目(2009AA6BN078) （2009AA6BN078）
