生命科学研究2011,Vol.15Issue(2):176-183,8.
橡胶树内珠被培养研究进展
Advances in Study on Inner Integument Culture of Hevea brasiliensis
摘要
Abstract
Self-rooting juvenile clone in Hevea brasiliensis was a new kind of planting material which was established in the late 1970s, combined the merits of seedlings and budded clones, and had a characteristic of fast-growing, high yield, large conical angle, was very likely to replace the budded clones as the third generation of planting material. Inner integument culture was the important approach to obtain self-rooting juvenile clone, regenerating plants by primary somatic embryogenesis and maintained somatic embryogenesis. Plants regenerated by primary embryos or microcutting grew more vigorously, produced higher rubber yield than the control, but showing low regeneration frequency; maintained somatic embryogenesis had high regeneration frequency, but regenerated/microcutting plants were abnormal, showing that they have poorer performance in growth, yield and disease resistance than the mature budded control. Researches on using maintained somatic embryogenesis on cryopreservation and genetic transformation was succeeded.关键词
橡胶树/内珠被/直接体胚发生/持续体胚发生Key words
Hevea brasiliensis/ inner integument/ primary somatic embryogenesis/ maintained somatic embryogenesis分类
农业科技引用本文复制引用
黄天带,孙爱花,周权男,戴雪梅,黄华孙..橡胶树内珠被培养研究进展[J].生命科学研究,2011,15(2):176-183,8.基金项目
国家自然科学基金资助项目(30960322) (30960322)
现代农业产业技术体系建设专项(NYCYTX-34) (NYCYTX-34)
海南省自然科学基金资助项目(807043) (807043)