山西大学学报(自然科学版)2011,Vol.34Issue(2):301-306,6.
四膜虫有性生殖过程中特异表达基因TCD3的序列分析
Sequence Analysis of Conjugation Specific Expressed TCD3 from Tetrahymena thermophila
摘要
Abstract
The protein which contains CHROMO(chromatin organization modifier)domain participates the regulation of chromatin structure.Conjugation specific expressed TCD3(Tetrahymena chromo domain-containing protein 3)gene was obtained by bioinformatics from Tetrahymena genome database( www.ciliate.org).The microarray data showed TCD3 gene was conjugation specific expressed.The ORF of TCD3 contained 1005 bp,and the macronuclear chromosome counterpart fragment was 1241 bp including two introns.5'RACE showed 5'untranslated region(5' UTR)of TCD3 contains 40 bp,3'RACE showed 3'untranslated region(3' UTR)of TCD3 contains 90 bp.Deduced Tcd3 protein encoded 334 amino acids and included one CHROMO domain.The cluster analysis of CHROMO domain implies Tcd3 might involved in the heterochromatin formation and DNA elimination.关键词
CHROMO结构域/四膜虫/TCD3基因/RACE/聚类分析分类
生物科学引用本文复制引用
张理光,王清路,朱雅静,王伟..四膜虫有性生殖过程中特异表达基因TCD3的序列分析[J].山西大学学报(自然科学版),2011,34(2):301-306,6.基金项目
国家自然科学基金(30770295 ()
31072000) ()
山西省自然科学基金(2008011063) (2008011063)
山西省高等学校优秀青年学术带头人支持计划资助项目 ()