南方医科大学学报2011，Vol.31Issue(5)：755-760,6.DOI:CNKI:44-1627/R.20110325.1055.001
一种斑马鱼原始造血髓系细胞突变体的研究
Study of a new zebrafish mutant defective in primitive myelopoiesis
摘要
Abstract
Objective To perform phenotypic identification and characteristic analysis of a new zebrafish mutant 1276 defective in primitive myelopoiesis. Methods The AB strain male zebrafish were mutagenized with N-ethyl N-nitrosourea (ENU) to induce mutations in the spermatogonial cells, and the mutations were transmitted to the offsprings. The F3 embryos were screened by neutral red staining for identifying the mutants defective in primitive myelopoiesis. One of the myeloid mutants 1276 was further studied by cytochemistry and whole mount in stiu hybridization (WISH) with different lineage markers. Results A total of 2140 mutagenized genomes from the 12% F2 families were analyzed, and 12 mutants were identified to show abnormal signal by neutral red staining. In the primitive hematopoiesis stage, the mutant 1276 showed the absence of neutral red staining-positive cells in the whole body. The expression of microglia marker apoe was totally lost in the head of the mutant, and the expression of the macrophage marker l-plastin was slightly decreased in the head and remained normal in the ventral dorsal aorta region, but the granulocytes and erythrocytes developed normally, in the definitive hematopoiesis stage, the mutant 1276 still showed abnormal macrophages as found in the primitive hematopoiesis stage, but the granulocytes, erythrocytes and lymphocytes appeared normal. Conclusion The zebrafish mutant 1276 shows abnormalities in the function, development and migration of the macrophages in the primitive hematopoiesis stage, which can not be compensated in the definitive hematopoiesis stage.关键词
斑马鱼突变体/N-乙基-N-亚硝基脲诱变筛选/中性红/原始髓系造血/巨噬细胞/小胶质细胞Key words
zebrafish mutant/N-ethyl N-nitrosourea mutagenesis screens/neutral red /primitive myelopoiesis/macro-phages/microglia分类
生物科学引用本文复制引用
颜广,孟萍,许孟畅,温子龙,张文清,刘伟,戴朝霞,王鹍 ,刘靖,赵灵凤,黄志斌,陈小辉,马宁..一种斑马鱼原始造血髓系细胞突变体的研究[J].南方医科大学学报,2011,31(5):755-760,6.基金项目
国家自然科学基金海外及港澳学者合作研究项目(30828020) （30828020）
