南方医科大学学报2011，Vol.31Issue(5)：822-824,3.
分子线性探针技术分析四川地区结核分枝杆菌耐药情况
GenoType MTBDRplus assay for rapid detection of rifampin and isoniazid resistance,in Mycobacterium tuberculosis in Sichuan
摘要
Abstract
Objective To explore the molecular and epidemic characteristics of rifampin (RFP) and isoniazid (INH) resistance of mycobacterium tuberculosis (MTB) in Sichuan. Methods GenoType · MTBDRplus Assay Gtplus was used to examine 68 clinical isolates of MTB and 105 clinical specimens for mutations in rpoB, katG and inhA genes related to RFP and INH resistance. Results Of the 151 valid tests obtained, 44 (29.14%) and 26 (17.22%) showed drug resistance and multidrug resistance, respectively. Resistance to RFP and INH was found in 21.85% (33/151) and 24.50% (37/151) of the samples, respectively. The most prevalent mutations were rpoB S531L, katG S315T1 and inhA C-15T. The multidrug resistance rate in the sputum specimens was significantly higher than that in the non-respiratory samples (19.35% vs 7.41%). Conclusion Drug-resistant, especially multidrug-resistant tuberculosis is highly prevalent in Sichuan. The multidrug-resistant bacteria most frequently show rpoB S531L combined with katG S315T1 mutations, suggesting the necessity of developing rapid clinical identification methods for drug-resistant MTB to control the spread of the resistant strains.关键词
结核分枝杆菌/耐多药结核分枝杆菌/基因型/利福平/异烟肼Key words
tuberculosis/tuberculosis, multidrug-resistant/genotype/isoniazid/rifampin分类
医药卫生引用本文复制引用
多丽娜,王婷婷,宋兴勃,谢轶,陆小军,范红,应斌武,王兰兰,张磊..分子线性探针技术分析四川地区结核分枝杆菌耐药情况[J].南方医科大学学报,2011,31(5):822-824,3.基金项目
卫生部2010-2012年临床学科重点项目 （）
国家自然科学基金(30871117) （30871117）
