南方医科大学学报2011，Vol.31Issue(5)：825-829,5.
骨髓间充质干细胞对免疫损伤卵巢的修复作用
Effect of mesenchymal stem cell transplantation on immunological injury of the ovary in mice
摘要
Abstract
Objective To investigate the effect of mesenchymal stem cell (MSC) transplantation in repairing ovarian injury in mice sensitized with porcine ovarian proteins. Methods Wild-type female mice with ICR background (6-8 weeks old) were divided randomly into groups A, B and C (n=12). In groups B and C, the mice were treated with the total protein extract from porcine ovary to induce immunological injury of the ovary, while those in group A received no treatment. MSCs-derived from GFP transgenc mice were transplanted into the mice of group C, and equal volume of PBS was injected intraperitoneally in mice of the other two groups. PCR was used to detect GFP gene in the genomic DNA of the ovaries to assess MSCs homing in the ovary, and the reparative effect of MSCs on ovarian injury was evaluated using HE staining and TUNEL analysis. Results After transplantation, the MSCs could reach the injured ovaries to promote the repair of the ovarian injury, resulting also in reduced apoptosis of the granulosa cells (GCs) in the injured ovaries. Conclusion MSCs transplantation can promote the recovery of the immunological injury of the ovary in mice, the mechanism of which may involve reduced apoptosis of the GCs.关键词
骨髓间充质干细胞/卵巢/免疫损伤/修复Key words
mesenchymal stem cells/ovary/immune-mediated injury/injury repair分类
医药卫生引用本文复制引用
李威娜,谢琪璇,秦俊文,黄伟,叶绍恩,罗峰,张春雪,肖銮娟..骨髓间充质干细胞对免疫损伤卵巢的修复作用[J].南方医科大学学报,2011,31(5):825-829,5.基金项目
国家自然科学基金(30370541) （30370541）
211工程3期生物科学技术与生物医学工程建设项目 （）
