南方医科大学学报2011，Vol.31Issue(5)：903-907,5.
转染IL-12基因的人脐带间充质干细胞对卵巢癌细胞的体外增殖和对裸鼠体内移植瘤的抑制作用
Human umbilical cord mesenchymal stem cells with adenovirus-mediated interleukin 12gene transduction inhibits the growth of ovarian carcinoma cells both in vitro and in vivo
赵雯红 1程建新 1史鹏飞 1黄军岩1
作者信息
- 1. 河北医科大学第四医院妇产科,河北,石家庄,050000
- 折叠
摘要
Abstract
Objective To study the inhibitory effect of human umbilical cord mesenchymal stem cells (UC-MSCs) infected by a adenoviral vector containing interleukin 12 (IL-12) gene on the proliferation of ovarian carcinoma SKOV3 in vitro and the growth of tumor explants in nude mice. Methods Cultured human UC-MSCs were infected with the recombinant adenovirus vector harboring IL-12 gene to establish the IL-12-€xpressing cell line AdIL-12-MSCs. Western blotting and RT-PCR were used to detect IL-12 expressions in AdIL-12-MSCs at the protein and Mrna levels, respectively. ELISA were used to detect IL-12 content in the supernatant of AdIL-12-MSCs, whose effect on the proliferation and apoptosis of ovarian carcinoma SKOV3 cells was evaluated with MTT assay and flow cytometry, respectively. In a nude mouse model bearing subcutaneous SKOV3 tumor explants, AdIL-12-MSCs were infused via the tail vein and the inhibitory effect on the tumor growth was observed. Results The exogenous IL-12 gene was successfully transduced into UC-MSCs by the recombinant adenovirus vector, resulting in efficient IL-12 expression in the cell at both the protein and Mrna levels. The supernatant of AdIL-12-MSCs significantly inhibited the proliferation of SKOV3 cells and induced cellular apoptosis in vitro as compared with UC-MSC supernatant. In the tumor-bearing nude mouse model, the transplantation of AdIL-12-MSCs significantly inhibited the growth of SKOV3 tumor explants (P<0.05). Conclusion Human UC-MSCs with IL-12 gene transduction, which express IL-12 at protein and Mrna levels, can inhibit the proliferation and induce apoptosis of ovarian carcinoma SKOV3 cells in vitro, and suppress the growth of ovarian cancer explants in nude mice.关键词
卵巢癌/人脐带间充质干细胞/IL-12/增殖/凋亡Key words
ovarian carcinoma/umbilical cord mesenchymal stern cells/IL-12/proliferation/apoptosis分类
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赵雯红,程建新,史鹏飞,黄军岩..转染IL-12基因的人脐带间充质干细胞对卵巢癌细胞的体外增殖和对裸鼠体内移植瘤的抑制作用[J].南方医科大学学报,2011,31(5):903-907,5.
