安徽农业科学2011，Vol.39Issue(6)：3604-3605,3614,3.
猪链球菌2型溶血素融合蛋白的制备及免疫活性测定
Preparation and Immunological Activity Analysis for the Gene Engineering Streptococcus suis Serotype 2 Suilysin Fusion Protein
摘要
Abstract
[ Objective ] To study human Streptococcus suis serotype 2 suilysin fusion protein and investigate its immunological activity. [ Method ]sly gene was obtained from ZYH33 ,which was isolated from human meningitis in Ziyang of Sichuan province. The gene was amplifiod by PCR with the template pMD18-T/sly. The gene fragments and expressing vector pQE-30 were ligated. The recombinant vector were transformed into TG1.The correct recombinatioas were identified by PCR,enzyme digestion and sequencing. Recombination protein and the peptides were detected by SDS-PAGE after induced by IPTG. The antigenicity of the recombination protein was determined with Western blot indicated by rabbit antiZY05719 strain polyclonal antibody. ［ Result ]The sly gene fragments could expressed and the products were recognized by rabbit-ati Streptococcus suis serotype 2 polyclonal antibody. [ Conclusion ] This work demonstrated that the fragment encoding 230 to 593 amino acid resides of suilysin could be an effective antigen candidate for Streptococcus suis diagnosis and may be useful in the development of diagnostic reagents and genetic engineering vaccines.关键词
猪链球菌2型/溶血素/克隆/表达/抗原性Key words
Streptococcus suis serotype 2/ Suilysin/ Clone/ Expression/ Antigenicity分类
农业科技引用本文复制引用
王海丽,徐公义,葛长城,赵德明..猪链球菌2型溶血素融合蛋白的制备及免疫活性测定[J].安徽农业科学,2011,39(6):3604-3605,3614,3.基金项目
山东省教育厅科技计划项目(J08LF57) （J08LF57）
2010年度高等学校优秀青年教师国内访问学者项目 （）
山东省聊城市科技攻关计划项目(20082048)资助. （20082048）
