核农学报2011，Vol.25Issue(2)：286-291,6.
电子束辐照对灰葡萄孢菌分生孢子萌发活性及致病力的影响
EFFECT OF ELECTRON BEAM IRRADIATION ON CONIDIAL GERMINATION ACTIVITY AND PATHOGENICITY OF Botrytis cinerea
摘要
Abstract
Conidia of Botrytis cinerea were irradiated by electron beam at 0. 5, 1.0, 2. 0 and 3.0kGy. The influence of electron beam on the activities of conidial germination and pathogenicity at the temperatures of 5℃ and 25℃ were tested, respectively. The results showed that the electron beam could inhibit germination of conidia and the length of germ tube of Botrytis cinerea, and delay the germination time. It could also decrease the pathogenicity obviously and higher irradiation dose showed stronger effects. Compared with control, the complete germination time of conidia extended to 5 and 9d at the cultivate temperatures of 25℃ and 5℃, after 2kGy of irradiation, and the germination rate was reduced 46. 57％ and 33.68％, respectively. The inhibition rates of germ tube were 25. 12％ and 74. 29％ when cultured 24h. The pathogenicity of Botrytis cinerae to strawberry was reduced significantly. After 2. 0kGy irradiation and cultivate at 25℃ for 2d, the disease index was 4. 17 and it decreased to 15.28 after cultivation of 5℃ for 15d. Electron beam treatment could inhibit the spore germination and germ tube elongation of Botrytis cinerea significantly, delayed the germination time, and reduced its pathogenicity, the higher the dose, the effect was more obvious.关键词
电子束/灰葡萄孢菌/分生孢子/萌发/致病力Key words
electron beam/ Botrytis cinerea/ conidia/ germination/ pathogenicity引用本文复制引用
张婷,陈召亮,乔勇进..电子束辐照对灰葡萄孢菌分生孢子萌发活性及致病力的影响[J].核农学报,2011,25(2):286-291,6.基金项目
上海市科技兴农重点项目(沪农科攻字(2008)第10-6号),上海市科委国际合作计划项目(073907003) （沪农科攻字(2008）
