核农学报2011，Vol.25Issue(2)：363-368,6.
PEG模拟干旱胁迫对石斛DNA表观遗传变化的MSAP分析
MSAP ANALYSIS OF EPIGENETIC CHANGES IN Dendrobium huoshanense UNDER PEG SIMULATED DROUGHT STRESS
摘要
Abstract
The methylation-sensitive amplified polymorphism (MSAP) techniques were used to assess genetic and epigenetic variations of Dendrobium huoshanense under drought stress. Results showed that the level of global DNA methylation decreased with increasing of PEG-6000 concentrations. When the tube seedlings were treated with 5％ ,10％, 15％ of PEG-6000, the levels of DNA methylation dropped to 34.7％, 32.9％ and 30.4％, respectively,compared with that for the control group ( 37. 2％ ). There was a significantly negative correlation ( r = - 0. 998 )between PEG-6000 and DNA methylation. In contrast with the control, under stress of 5％, 10％ and 15％ PEG-6000,methylation and demethylation of DNA were 12. 02％, 12. 50％, 15.53％ and 8.39％, 8.55％ , 8.45％, respectively.The ratios of DNA methylation and demethylation, the polymorphism of DNA methylation increased gradually,accompanied with the enhancement of drought stress.关键词
霍山石斛/甲基化敏感扩增多态性/干旱胁迫/DNA甲基化Key words
Dendrobium huoshanense/ MSAP/ drought stress/ DNA methylation引用本文复制引用
樊洪泓,李廷春,李正鹏,林毅,蔡永萍,金青..PEG模拟干旱胁迫对石斛DNA表观遗传变化的MSAP分析[J].核农学报,2011,25(2):363-368,6.基金项目
安徽省自然科学基金青年基金项目(10040606Q22),安徽省高等学校优秀青年人才基金项目(2011SQRL053),安徽省教育厅重点自然科学项目(KJ2011A109),校稳定人才基金项目(yj2008-27),校长青年基金项目,安徽省中药材产业体系 （10040606Q22）
