医学分子生物学杂志2011，Vol.8Issue(3)：240-244,5.DOI:10.3870/j.issn.1672-8009.2011.03.011
人FAM92A1基因诱饵表达质粒的构建与鉴定
Construction and Identification of Bait Expression Plasmid of Human FAM92A1
摘要
Abstract
Objective To construct the bait expression plasmid pGBKT7-hFAM92A1 and detect its protein expression, toxicity and self-activation. Methods The sequence of human FAM92A1was amplified by PCR, and then was cloned into pGBKT7. After verified by cutting and sequencing,the constructed pGBKT7-hFAM92A1 plasmid was transformed into yeast cells AHl09, and the expression of the bait protein Was analyzed by Western Blot. Toxicity and self-activation of the bait protein were detected. Results The sequence of human FAM92A1 was amplified and cloned into pGBKT7 successfully. Transformation of the bait expression plasmid into yeast cells AHl09 did not have self-activating effect. Expression of the bait protein was confirmed by Westem Blot, showing no toxicity to yeast AH109 cell. Conclusion The construct of pGBKT7-hFAM92A1 can correctly encode human hFAM92A1 protein in yeast cells, and can be used as a bait in yeast two-hybrid system.关键词
hFAM92A1/诱饵表达质粒/诱饵蛋白Key words
human FAM92A1 / bait expression plasmid/ bait protein分类
生物科学引用本文复制引用
方娟,李瑞明,卢智勇,孙晓东,陈洁,阮绪芝..人FAM92A1基因诱饵表达质粒的构建与鉴定[J].医学分子生物学杂志,2011,8(3):240-244,5.基金项目
湖北省自然科学基金(No.2010CDB09102) （No.2010CDB09102）
