安徽农业科学2011，Vol.39Issue(6)：3234-3236,3.
獐牙菜苦苷对H2O2诱导大鼠心肌细胞凋亡的保护作用
Protective Effect of Swertiamarin on H2O2-Induced Apoptosis of Rat Cardiomyocyte
徐仙赟
1程齐来 2李洪亮 2苏水莲1
作者信息
- 1. 赣南医学院基础医学院,江西赣州,341000
- 2. 赣南医学院药学院,江西赣州,341000
- 折叠
摘要
Abstract
[ Objective ] To observe the protective effect of swertiamarin on H2O2-induced apoptosis of rat' s cardiomyocyte and to explore its protective mechanism. [ Methods] Cultured with differential attachment methods, the cell viability and content of Superoxide dismutase(SOD) and Malondia ldehyde MDA of SD rat' s cardiomyocyte of were determined; the expression of bcl-2 and Bax of cardiomyocyte was detected with immunocytochemical staining and the cell apoptosis was detected using acridine orange staining. [ Results ] The rats given swertiamarin of three different concentrations was higher in survival rate of cardiomyocyte and SOD, and was lower in MDA, and was significant different from that in H2O2-induced group ( P ＜ 0.05 ; P ＜ 0.01 ). [ Conclusion] Swertiamarin is endowed with protective effect on H2O2-induced apoptosis of rat's cardiomyocyte, which may be acted through influencing the oxidation system and regulating and controlling specific proteins of cardiomyocyte.关键词
獐牙菜苦苷/氧化应激/心肌细胞/凋亡/保护Key words
Swertiamarin/ Oxidative stress/ Cardiomyocyte/ Apoptosis /Protection分类
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徐仙赟
,程齐来,李洪亮,苏水莲..獐牙菜苦苷对H2O2诱导大鼠心肌细胞凋亡的保护作用[J].安徽农业科学,2011,39(6):3234-3236,3.
