摘要
Abstract
Objective To detect the expression of p-MAPK, CyclinD1 and CDK4 proteins in human colorectal carcinomas, and explore their correlations. Methods Seventy-eight saucples of colorectal carcinoma tissues were intraoperatively obtained, and stained with HE for pathomorphologic examination. p-MAPK, CyclinD1 and CDK4 were detected in the 78 colorectal carcinoma,tumor adjacent colorectal carcinoma tissues and normal tissues by immunohistochemical method and RT-PCR. Results Positive expression rates for p-MAPK, CyclinD1 and CDK4 proteins in colorectal carcinoma were 67. 9 ％ (53/78) , 57. 7 ％ (45/78 ) and 39. 7 ％ (31/78 ) and in the tissues adjacent to tumors were 6. 4 ％ (5/78) , 10. 3 ％ ( 8/78) and 19. 2％% ( 15/78) , respectively. Positive expression rates and levels of p-MAPK, CyclinDI and CDK4 protein in carcinoma were significantly higher than those in the mucosa (P ＜ 0. 01) . Significant correlation between the expression of p-MAPK and CyclinDI was found ( P ＜ 0. 01 ) . There were no significant correlations between the expression of p-MAPK, CyclinDI and CDK4 proteion (P ＞ 0. 05 ) . The correlation between the expression of CyclinDI and CDK4 was found ( P ＜ 0. 05 ) . The results of immunohistochemistry, RT-PCR indicated that positive expression rates for p-MAPK, CyclinDI and CDK4 proteins in colorectal carcinoma were 8. 2 ％ ( 61/78 ) , 67. 9 ％ (53/78 ) and 53. 8 ％ ( 42/78 ) ,and in the tissues adjacent to tumors were 3. 8 ％ ( 3/78) 、 2. 6 ％ ( 2/78) and 6. 4 ％ (5/78) .Conclusion The results indicate that MAPK may play an important role in the pathogenesis of colorectal carcinoma. The Cvclin D1 and cdk complex can promot cell cycle progression.关键词
p-MAPK/CyclinD1/CDK4/大肠癌/免疫组织化学Key words
p-MAPK/ cyclinDI / CDK4 / colorectal carcinoma/ immunohistochemical分类
医药卫生
