安徽农业科学2011，Vol.39Issue(6)：3260-3261,2.
鱼腥草中槲皮素含量分析方法研究
Study on the Analysis Methods of Quercettn Content in Houttuynia cordata Thunb
赵国文 1张丽萍 1龚靖2
作者信息
- 1. 四川理工学院材料与化学工程学院,四川自贡,643000
- 2. 成都龙泉高科天然药业有限公司,四川成都,610100
- 折叠
摘要
Abstract
[ Objective ] The aim was to establish a HPLC method for the content determination of natural quercetin in different parts of Houttuynia cordata Thunb. [ Methods ] The combination between the cellulase and ultrasonic extracting was used; and the chromatographic column was XDBC18 (4. 6 mm × 150 mm,5 μm) ,mobile phase was acetonitrile - 1％ acetic acid (40∶ 60) ,column temperature was 25 ℃ ,and the flow velocity was 0. 8 ml/min,the detection wavelength was 370 nm. [ Result ] There was good linear relationship for linear within the range of 0. 1 - 14.4 μg/ml (r =0.999 9) and the average recovery of quercetin in the roots of Houttuynia cordata Thunb samples was 99.2％ and RSD was 3.9％.The average recovery of quercetin in the stems and leaves of Houttuynia cordota Thunb was 98.8％ and RSD was 3.8％. [ Conclusion ]The established methods had many advantages, such as effective separating effect, speciality, simple operation, accurate results and good reproducibility,therefore,it can be applied for the determination of quercetin in Houttuynia cordata Thunb.关键词
高效液相色谱法/鱼腥草/槲皮素/测定Key words
HPLC/Houttuynia cordata Thunb/ Quercetin/ Determination分类
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赵国文,张丽萍,龚靖..鱼腥草中槲皮素含量分析方法研究[J].安徽农业科学,2011,39(6):3260-3261,2.
