中国医学科学院学报2011，Vol.33Issue(2)：185-188,4.DOI:10.3881/j.issn.1000-503X.2011.02.017
系统性红斑狼疮患者外周血单个核细胞中微小RNA-146a的表达
Expression of MicroRNA-146a in Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus
摘要
Abstract
Objective To explore the expression pattern of microRNAs in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus ( SLE), with an attempt to identify the role of microRNA in the pathogenesis of SLE. Methods SLE-related genes were searched from the published literatures. Using the microRNA target gene prediction databases, we predicted the putative microRNA targets in these SLE-related genes. For some of the corresponding microRNAs (hsa-miR-146a) , quantitative real-time polymerase chain reaction was performed to determine the expression levels of these microRNAs in PBMCs of SLE patients (SLE group) and healthy controls (control group). Result The discrepancy of cycle threshold of hsa-miR-146a in PBMCs was significantly higher in SLE group (4.52 ± 1.18) than in control group (2.76 ± 1.38 ) ( P - 0.02), and the expression level of hsa-miR-146a was significantly lower in SLE group. Conclusion The expression of hsa-miR-146a decreases in SLE patients, indicating that hsa-miR-146a may play a role in the pathogenesis of SLE.关键词
微小RNA/系统性红斑狼疮/外周血单个核细胞Key words
microRNA/ systemic lupus erythematosus/ peripheral blood mononuclear cells分类
医药卫生引用本文复制引用
王海燕,李扬,陈梅红,张烜..系统性红斑狼疮患者外周血单个核细胞中微小RNA-146a的表达[J].中国医学科学院学报,2011,33(2):185-188,4.基金项目
教育部新世纪优秀人才计划(NCET-04-0191),国家自然科学基金(30400410),北京自然科学基金(7052052)和国家重点基础研究发展计划项目子课题(2007CB512405) （NCET-04-0191）
