中国医学科学院学报2011，Vol.33Issue(2)：205-209,5.DOI:10.3881/j.issn.1000-503X.2011.02.021
胰蛋白酶原激活肽和白细胞介素-6在实验性急性胰腺炎中的意义
Significance of Trypsinogen Activation Peptides and Interleukin-6 in Experimental Acute Pancreatitis
高军 1田志军 1邱宝樑 1李非 2孙家邦2
作者信息
- 1. 北京市第六医院普外科,北京,100007
- 2. 首都医科大学附属宣武医院普外科,北京,100053
- 折叠
摘要
Abstract
Objective To explore the feasibility of using plasma trypsinogen activation peptides (TAP) and serum interleukin-6 (IL-6) as early markers for predicting the severity of experimental acute pancreatitis. Methods Ninety male adult Sprague-Dawley rats were equally randomized into five groups: edema pancreatitis group, treated with retrograde ductal infusion of 3 ％ sodium taurocholate solution; necrosis pancreatitis group, treated with retrograde ductal infusion of 5％ sodium taurocholate solution; treatment pancrcatitis group, treated with retrograde ductal infusion of 3 ％ sodium taurocholate solution and ulinastatin intravenous infusion half an hour later; control pancreatitis group, treated with 0.9％ normal saline retrograde ductal infusion; and sham operation group, treated with sham operation. Rats in each group were equally randomized into three subgroups, which were killed by exsanguination 3, 6, or 24 hours after infusion, and blood specimens were obtained. Serum amylase, plasma TAP, and serum IL-6 were determined. The severity of pancreatitis was scored by two blinded pathologists under microscope. Results At 3 and 6 hours after infusion, plasma TAP concentration of necrosis pancreatitis group [ (4. 798 ± 0. 169 ) and ( 3. 999 ± 0. 299 ) nmol/L, respectively ] were significantly higher than those of edema pancreatitis group [ ( 2.416 ± 0. 148 ) and ( 3. 356 ± 0.211 ) nmol/L, respectively ] ( P ＜ 0.01 ); at 6 hours after infusion, serum IL-6 level of necrosis pancreatitis group [ (1339.51 ±56.43) pg/ml] was significantly higher than that of edema pancreatitis group [ (619.07 ± 42.25 ) pg/ml ] ( P ＜ 0.01 ). Conclusions In this acute pancreatitis model, the peak levels of plasma TAP and serum IL-6 may appear earlier in rats with severer disease. Serum TAP level may be used as a marker for the accurate early prediction of the severity of acute pancreatitis.关键词
胰蛋白酶原激活肽/白细胞介素-6/急性胰腺炎/胰酶抑制剂Key words
trypsinogen activation peptides/ interleukin-6/ acute pancreatitis/ pancreatin inhibitor分类
医药卫生引用本文复制引用
高军,田志军,邱宝樑,李非,孙家邦..胰蛋白酶原激活肽和白细胞介素-6在实验性急性胰腺炎中的意义[J].中国医学科学院学报,2011,33(2):205-209,5.
