中国医学科学院学报2011，Vol.33Issue(2)：210-213,4.DOI:10.3881/j.issn.1000-503X.2011.02.022
三氯醋酸-丙酮沉淀法用于骨组织蛋白的提取
Application of Trichioroacetic Acid-Acetone Precipitation Method for Protein Extraction in Bone Tissue
摘要
Abstract
Objective To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid (TCA) -acetone precipitation method.Methods Hydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction.The efficiencies of these two methods were compared.Results TCA-acetone precipitation method had significantly higher extraction efficiency.Compared with HCL decalcification method, it had less pollution from bone marrow hematopoietic tissue.Protein band distribution was similar between these two methods.Conclusion TCA-acetone precipitation method is useful for bone proteomics research.关键词
三氯醋酸-丙酮沉淀法/骨组织/蛋白提取Key words
trichloroacetic acid-acetone precipitation method/ bone tissue/ protein extraction分类
生物科学引用本文复制引用
崔永锋,杨贞,朱宝华,肖鲁伟,童培建..三氯醋酸-丙酮沉淀法用于骨组织蛋白的提取[J].中国医学科学院学报,2011,33(2):210-213,4.基金项目
中国博士后科学基金(20080431334) （20080431334）
