南方医科大学学报2011，Vol.31Issue(4)：619-623,5.
间歇性缺氧时结肠癌细胞的晚期氧化蛋白产物与血管内皮生长因子、转化生长因子β1的关系
Relation of advanced oxidation protein products with VEGF and TGF-β1 in colon cancer cells exposed to intermittent hypoxia
摘要
Abstract
Objective To investigate the association of advanced oxidation protein produts (AOPP) with oxidative stress in colon cancer cells exposed to intermittent hypoxia (IH). Methods Colon cancer SW480 cells were exposed to IH, continuous hypoxia, or normoxia. Enzyme-linked immunosorbent assay (ELISA) was employed to examine the levels of AOPP and vascular endothelial growth factor (VEGF), xanthine oxidase assay was used to determine malonaldehyde (MDA) and glutathione peroxidase (GSH-PX), and Western blotting and immunofluorescence assay were performed for detection of transforming growth factor-β1 (TGF-β1) expression. Results Compared with the normoxia group, the two hypoxia groups showed significantly increased AOPP and MDA levels (P＜0.05) and lowered SOD and GSH-PX levels (P＜0.05). The concentration of AOPP was positively correlated to MDA, VEGF, and TGF-p\ levels (P＜0.05), but inversely to SOD. No significant correlation was found between AOPP and GSH-PX levels. Conclusion Compared with continuous hypoxia, IH results in more obvious protein oxidation in relation to oxidative stress. The increased expression of VEGF and TGF-β1 in the context of hypoxia is closely related to AOPP level.关键词
间歇性缺氧/氧化应激/晚期氧化蛋白产物/血管内皮生长因子/转化生长因子β1Key words
intermittent hypoxia/ oxidative stress/ advanced oxidation protein products/ vascular endothelial growth factor/ transforming growth factor-p\分类
医药卫生引用本文复制引用
冼乐武,李涛平,韦伊尔,伍思培,马磊..间歇性缺氧时结肠癌细胞的晚期氧化蛋白产物与血管内皮生长因子、转化生长因子β1的关系[J].南方医科大学学报,2011,31(4):619-623,5.基金项目
国家自然科学基金(30471720) （30471720）
