南方医科大学学报2011，Vol.31Issue(4)：628-632,5.
高表达Ck2β与大肠癌发生和转移的相关性研究
Correlation of casein kinase 2β overexpression to the metastatic ability of colorectal cancer cells in vitro
摘要
Abstract
Objective To investigate the expression of casein kinase 2β (ck2β) in colorectal cancer in relation to the metastatic ability of the cancer cells. Methods The expression of ck2p in 46 normal colorectal mucosa, 20 colorectal adenomas and 66 colorectal cancers were detected immunohistochemically. In colorectal cancer cells, Ck2p protein expression was knockdowned by RNA interference using ck2p-specific small interfering RNA (siRNA) and the interference efficiency was assessed by Western blotting. The effect of ck2p gene knockdown on the proliferation of the colorectal cancer cells was tested with colony formation assay, and the changes in the invasive ability of the cells were observed using Transwell chamber assay. Results Negative or weak ck2p expression was detected in normal colorectal mucosa, with nuclear posihvity in 8.7% and cytoplasmic positivity in 13.0% of the cases. Colorectal adenomas showed moderate ck2p expression, with 60% cases showing positivity in the cell nuclei and 40% in the cytoplasm. In colorectal cancers, significantly stronger expression of ck2p was found than that in colorectal adenomas and normal colorectal mucosa (.(P＜0.05), and 75.8% cases showed positivity in the cell nuclei and 62.1% showed cytoplasmic positivity; the expression of ck2p protein in colorectal cancers with lymph node metastasis was even higher (P＜0.05). Ck2p knockdown obviously inhibited the proliferation and invasiveness of colorectal cancer cells in vitro. Conclusion The high expression of ck2β in colorectal cancer is closely correlated to the carcinogenesis and metastasis of the tumor.关键词
ck2β/大肠癌发生/大肠癌转移Key words
casein kinase 2β/ colorectal cancer/ colorectal mucosa/ colorectal adenoma分类
医药卫生引用本文复制引用
邹金金,罗何三,黄志勇,董忠谊,曾钦,吴德华..高表达Ck2β与大肠癌发生和转移的相关性研究[J].南方医科大学学报,2011,31(4):628-632,5.基金项目
国家自然科学基金(30672312) （30672312）
广东省自然科学基金(10151051501000105) （10151051501000105）
