南方医科大学学报2011，Vol.31Issue(4)：649-652,4.
红景天苷抑制模拟微重力诱导的肺微血管内皮细胞凋亡
Salidroside inhibits clinorotation-induced apoptosis in pulmonary microvascular endothelial cells
摘要
Abstract
Objective To investigate the inhibitory effect of salidroside (Sal) on pulmonary microvascular endothelial cell (HPMEC) apoptosis induced by simulated microgravity and its mechanism. Methods Human pulmonary microvascular endothelial cells cultured in vitro were divided into control group, clinorotation group and clinorotation+Sal pretreatment groups. Microgravity was simulated by clinorotation. The apoptotic rate of HPMECs was detected by flow cytometry using Annexin V-FITC staining, and the expressions of bcl-2, bax, and caspase-3 at the mRNA and protein levels were determined by real-time PCR and Western blotting, respectively. Results A 72-h clinorotation significantly induced apoptosis in HPMECs. Real-time PCR results demonstrated a significantly lowered bcl-2 but increased bax and caspase-3 mRNA expressions in clinorotation group as compared with those in the control group. Western blotting showed that clinorotation inhibited the protein expressions of PI3K and p-AKT and increased caspase-3 protein expression. Salidroside significantly inhibited the cell apoptosis, reversed the expressions of Bcl-2 and Bax, and attenuated the decrease in the protein expression of PI3K and phosphorylation level of AKT. Salidroside also antagonized the activation of caspase-3. Conclusion PI3K/AKT pathway and caspase 3 are involved in the apoptosis of HPMVECs induced by clinorotation, and the effect of clinorotation can be reversed by salidroside, suggesting the potential value of salidroside for application in spaceflight.关键词
红景天苷/模拟微重力/微血管内皮细胞/细胞凋亡Key words
salidroside/ simulated microgravity/ microvascular endothelial cells/ apoptosis分类
医药卫生引用本文复制引用
康春燕,李婷,邹琳,袁明,李天志,郭英华,王洋,刘长庭..红景天苷抑制模拟微重力诱导的肺微血管内皮细胞凋亡[J].南方医科大学学报,2011,31(4):649-652,4.基金项目
军队医药卫生十一·五计划专项滚动课题(06Z048) （06Z048）
