福建农业学报2011，Vol.26Issue(1)：10-12,3.
鸭出血性卵巢炎病毒RT-PCR检测方法的建立
Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt
摘要
Abstract
A pair of primers based on NS5 gene sequences of different strains of genus Flavivirus was designed for amplification of 470 bp fragments. No positive target fragment was available from H9 subtype influenza virus,Muscovy duck reovirus, duck plague, duck hepatitis virus (DHV-I ) or duck-origin avian paramyxovirus type Ⅰ.The detection limit reached 20 pg under the optimized conditions. The results showed that the RT-PCR was sensitive, specific and applicable for rapid laboratory diagnosis and epidemiologic surveillance.关键词
鸭出血性卵巢炎/黄病毒/RT-PCRKey words
ducks hemorrgagic ovaritis/ Flavivirus/ RT-PCR分类
农业科技引用本文复制引用
万春和,施少华,程龙飞,陈红梅,傅光华,彭春香,林芳,林建生,黄瑜..鸭出血性卵巢炎病毒RT-PCR检测方法的建立[J].福建农业学报,2011,26(1):10-12,3.基金项目
"十二五"国家水禽产业技术体系(CARS-43-01A) （CARS-43-01A）
科技部科技人员服务企业行动项目(2009GJC40037) （2009GJC40037）
福建省星火科技计划项目(2009S0110) （2009S0110）
福建省农业科学院科技创新团队建设基金(STIF-Y02) （STIF-Y02）
