福建农业学报2011，Vol.26Issue(1)：19-23,5.
人工感染嗜水气单胞菌对日本鳗鲡非特异性免疫功能的影响
Effects of Aeromonas hydrophila on nonspecific immunity of Anguilla japonica in a challenge experimentation
摘要
Abstract
Eels (Anguilla japonica) were randomly divided into the infected and control groups. Fish in the infected group were intraperitoneally injected with Aeromonas hydrophila suspension in the concentration of 3 × 106 CFU · mL-1 , while those in the control group the same amount of sterile saline solution (0. 85％ NaCI). The eels were sacrificed to determine their nonspecific immunity on parameters including haematocyte count (i. e.,erythrocyte, leucocyte, lymphocyte and granulocyte), leucocyte phagocytic activity and serum antibacterial activity. The results showed that (a) the A. hydrophila infection increased the numbers of leucocytes and lymphocytes in the peripheral blood of A. japonica, and significantly reduced the number of granulocytes; (b) the phagocytic index and phagocytic rate increased on the Ist day after injection, and were very significantly different (P ＜0.01) or significantly different (P＜0. 05) on the 5th day and 9th day, as compared with the control; (c) on the Ist day after injection, the antibacterial activity of the infected fish also increased, and on the 9th day and 13th day, it was very significantly different (P＜0. 01) from the control. The results suggested that the intraperitoneal injection enhanced A. japonica ’s resistance to the invading A. hydrophila by means of lymphocyte, especially, the proliferation of leucocytes, the increase in leucocyte phagocytic activity and the excretion of antibacterial substances.关键词
日本鳗鲡/嗜水气单胞菌/外周血相/吞噬活性/抗菌活力/非特异性免疫Key words
Anguilla japonica/ Aerononas hydrophila/ peripheral blood/ phagocytic activity/ antibacterial activity/ nonspecific immunity分类
农业科技引用本文复制引用
张伟妮,刘巧红,陈其世,姜玮,王寿昆,王全溪,林旋..人工感染嗜水气单胞菌对日本鳗鲡非特异性免疫功能的影响[J].福建农业学报,2011,26(1):19-23,5.基金项目
福建省自然科学基金(2008J0250) （2008J0250）
