安徽农业科学2011，Vol.39Issue(7)：4004-4006,3.
亮甲酚蓝染色对水牛卵母细胞体外受精和孤雌激活胚胎发育的影响
Effects of Brilliant Cresvl Blue Staining on the Efficiency ofin vitro fertilization and Parthenogenetic Activation of Oocytes in Buffalo
摘要
Abstract
[ Objective ] This paper aims to evaluate the effects of brilliant cresyl blue (BCB) staining on the efficiency of in vitro fertilization ( IVF)and parthenogenetic activation(PA) of oocytes in buffalo. [ Method] Cumulus oocyte complexes (COCs) are stained with BCB (26 μ mol/L) for 90 min before in vitro maturation (IVM). Subsequently, COCs are divided into BCB-group (colorless cytoplasm) and BCB +group (colored cytoplasm) according to their ooplasm BCB coloration. The COCs without BCB staining are considered as control group, and the COCs of holding control group are incubated in DPBS supplemented with 0. 4％ BSA for 90 min. After IVM, the maturation rate, embryo development rate of IVF and PA in each group are evaluated. [ Result] The maturation rate in BCB + group(65.70％ ) is significantly higher than that of control group (59.86％), holding control group (58.42％) and BCB-group (48.86％). The blastocyst rate of IVF in BCB +group (27.08％) is significantly higher than that of holding control group (20.50％) and BCB-group( 8.45％ ), but there is no significant difference between the BCB + group and the control group( 27.08％ vs 25.49％ ). There is no significant difference in parthenogenetic blastocyst rate among the BCB + group, control group and holding control group( 19.13％ ), but all of the three groups are significantly higher than that of BCB-group. [ Conclusion] The positive buffalo COCs selected by BCB staining before IVM have superior developmental competence in maturation, but it could not significantly enhance the developmental rate of the IVF and PA embryos.关键词
水牛卵母细胞/体外受精/孤雌激活/亮甲酚蓝Key words
Buffalo oocyte/ IVF/ PA/ Brilliant cresyl blue分类
农业科技引用本文复制引用
黄怡,陈焕华,刘宏毅,张明,陆阳清,卢克焕..亮甲酚蓝染色对水牛卵母细胞体外受精和孤雌激活胚胎发育的影响[J].安徽农业科学,2011,39(7):4004-4006,3.基金项目
国家高技术研究发展计划(863计划)项目(2008AA1010-04) （863计划）
广西科学研究与技术开发项目(桂科攻0815008-2-4). （桂科攻0815008-2-4）
