安徽农业科学2011，Vol.39Issue(7)：4012-4014,3.
山羊核移植胚胎干细胞的分离培养
Isolated Culture of Nuclear Transplantation Embryonic Stem Cells of Goat
摘要
Abstract
The isolated cultures of nuclear transplantation embryonic stem cells (NT-ESCs) from nuclear transplantation embryos of goats in different developmental stages were compared. The results showed that NT-ESCs could be isolated from nuclear transplantation embryos of goats in all developmental stages, among which the cloning efficiency of NT-ESCs from hatching embryos was highest, being 38％; it had significant difference from that of blastula, which was 26％ and it had extremely significant difference from that of embryotic sphere, which was 18％. The shape of isolated NT-ESCs was similar to that of normal ESCs, both of them had alkaline phosphatase activities and could express pluripotency gene Oct-4 and surface marker antigen SSEA-1.关键词
山羊/核移植/胚胎干细胞Key words
Goat/ Nuclear transplantation/ Embryonic stem cells分类
农业科技引用本文复制引用
葛秀国,李吉霞,窦忠英..山羊核移植胚胎干细胞的分离培养[J].安徽农业科学,2011,39(7):4012-4014,3.基金项目
国家高技术研究与发展项目(2005AA219050). （2005AA219050）
