安徽农业科学2011，Vol.39Issue(6)：3649-3651,3.
气相色谱-质谱法测定食品中的反式脂肪酸
Test of the Trans Fatty Acid in Food with Gas-chromatography-Mass-spectrometry
摘要
Abstract
[ Objective ] 3 types of different trans-fatty acid:reversed palmitic acid,elaidic acid and antioleic acid were qualitatively and quantitatively analyzed with gas chromatography-mass spectrometry. [ Method ] The fat extracted from food was methylated under the condition of alkaline,isolated in the capillary column of Hp-INNOWax(30 m × 0.25 mm × 0. 25 μm) and detected with the mass spectrometry. The character of the sample was qualitatively identified with the CK from Standard Spectral Library based on the retention time of the standard sample and quanfitatively identified with the method of external standard. The content of trans-fatty acid in the sample was measured with a relevant formula. [ Results] The methyl esters of trans-fatty acid were better isolated with its isomer. There was good linearity (r ＞0. 999 5) in the concentration range of 0.001 -0.010 mg/ml,with the relative standard deviation of 0.648% -2.430% and the recovery of 89. 1% ～ 102.0%. The detection limit of the methyl esters of 3 types of different trans-fatty acids was 0. 800,0. 875 and 0. 840 μg/ml,respectively. [ Conclusion] This method was accurate,reliable and simple,which was suitable for the test of the main antieiso-fatty acid in food.关键词
反式脂肪酸/脂肪酸甲酯/气相色谱-质谱法/检测Key words
Trans-fatty acid/Fatty acid methyl esters/Gas chromatography-Mass spectrometry/Detection分类
农业科技引用本文复制引用
牛翠娇,史玉琴,殷志萍,赵文,宋涛..气相色谱-质谱法测定食品中的反式脂肪酸[J].安徽农业科学,2011,39(6):3649-3651,3.基金项目
河北农业大学新兴学科科研发展基金项目(FSY200661). （FSY200661）
