南方医科大学学报2011，Vol.31Issue(4)：653-655,3.
钠离子荧光探针用于检测雪卡毒素的细胞毒性
A cell-based detection of ciguatoxin using sodium fluorescence probe
摘要
Abstract
Objective To establish a cell-based detection method of ciguatoxin using fluorescence assay. Methods Mouse neuroblastoma N-2A cells were exposed to ouabain and veratridine and different concentrations of standard ciguatoxin samples (P-CTX-1) to establish the curvilinear relationship between the toxin dosage and fluorescence intensity using the sodium fluorescence probe CoroNa? Green. The toxicity curvilinear relationship was also generated between the toxin dosage and cell survival using CCK-8 method. Based on these standard curves, the presence of ciguatoxin was detected in 33 samples of deep-sea coral fish. Results A correlation was found between the detection results of cell-based fluorescence assay and cytotoxicity assay, whose detection limit reached 10"13 g/ml and 10-12 g/ml, respectively. The cell-based fluorescent assay sensitivity showed a higher sensitivity than cytotoxicity assay with a 2-4 h reduction of the detection time. Conclusions The cell-based fluorescent assay can quickly and sensitively detect ciguatoxin and may serve as a good option for preliminary screening of the toxin.关键词
雪卡毒素/钠离子荧光探针/荧光检测/细胞毒性Key words
ciguatoxin/ CoroNa Green/ fluorescence probe/ cytotoxicity分类
医药卫生引用本文复制引用
袁建辉,杨慧,唐焕文,黄薇,徐新云,刘建军,柯跃斌,程锦泉,庄志雄..钠离子荧光探针用于检测雪卡毒素的细胞毒性[J].南方医科大学学报,2011,31(4):653-655,3.基金项目
国家自然科学基金(30972500) （30972500）
广东省医学科研基金(A2008620) （A2008620）
深圳市科技计划项目(200602129) （200602129）
