南方医科大学学报2011，Vol.31Issue(4)：661-664,4.
类风湿关节炎患者滑液NK-22细胞对成纤维样滑膜细胞增殖的影响及其机制研究
Effect of NK-22 cells in the synovial fluid of patients with rheumatoid arthritis on the proliferation of fibroblast-like synoviocytes and its possible mechanism
摘要
Abstract
Objective To investigate the effect of NK-22 cells isolated from the synovial fluid (SF) of patients with rheumatoid arthritis (RA) on the proliferation of fibroblast-like synoviocytes and explore its possible mechanism. Methods The proportions of NK-22 cells in the peripheral blood (PB) and the SF of 20 RA patients and 20 healthy individuals were determined by flow cytometry. NK-22 cells in the SF sorted by flow cytometry were cultured for two weeks followed by a 4-h stimulation with 20 ng/ml phorbol 12-myristate 13-acetate and 0.5 junol/L ionomycin. The culture supernatant of NK-22 cells was then harvested, in which the levels of IL-22 and TNF-ct were measured by ELISA. The fibroblast-like synoviocytes were exposed to the culture supernatant for 24, 48, 72, and 96 h, and the changes in the cell proliferation were detected by MTT assay. Results RA patients showed a significantly greater proportion of NK-22 cells in both the SF and PB than the normal control subjects (P<0.05). NK-22 cells sorted by flow cytometry reached a purity exceeding 90%, and the levels of IL-22 and TNF-a in the culture supernatant of NK-22 cells cultured for two weeks were 941.16 pg/ml and 368.1 pg/ml, respectively. The culture supernatant of NK-22 cells caused a rapid proliferation of the fibroblast-like synoviocytes at 24, 48, 72, and 96 h after the exposure. Conclusion NK-22 cells in the SF of RA patients can promote the proliferation of fibroblast-like synoviocytes possibly due to the capacity of NK-22 cells to produce IL-22 and TNF-a.关键词
类风湿关节炎/NK-22细胞/成纤维样滑膜细胞/增殖Key words
rheumatoid arthritis/ NK-22 cell/ fibroblast-like synoviocyte/ proliferation分类
医药卫生引用本文复制引用
任洁,李娟,冯知涛,吕卓..类风湿关节炎患者滑液NK-22细胞对成纤维样滑膜细胞增殖的影响及其机制研究[J].南方医科大学学报,2011,31(4):661-664,4.基金项目
教育部博士点基金课题(20094433110006) （20094433110006）
