南方医科大学学报2011，Vol.31Issue(4)：674-677,4.
特异性miR-221抑制剂对结直肠癌细胞增殖及凋亡的影响
APPLICATION OF INTEGRATED GEOPHYSICAL TECHNIQUE IN BASEMENT STRUCTURE RESEARCH IN BINBEI AREA
摘要
Abstract
Objective To investigate miRNA-221 expression in human colorectal carcinoma (CRC) cells and the effects of miR-221-specific inhibitor on the proliferation and apoptosis of CRC cells. Methods Four human CRC cell lines (HT-29, Lovo, SW-480, and CaCO2) were examined for miRNA-221 expression using real-time Q-PCR. The specific 2-methoxy-modified RNA oligonucleotides of miR-221 (anti-miR-221) were synthesized and transfected into Caco2 cells via liposome, and the changes in the expression of miR-221 in the cells were detected by real-time Q-PCR. The proliferation and apoptosis of the transfected CRC cells were detected using MTT assay and flow cytometry. Results The 4 human CRC cells showed significantly upregulated expression of mtR-221 compare with HUVECs (P＜ 0.01). The miR-221-specific inhibitor, anti-miR-221, significantly inhibited the expression of miR-221 in Caco2 cells and suppressed the cell proliferation, causing also obvious cell apoptosis (P＜0.01). Conclusion The miR-221-specific inhibitor shows potent inhibitory effect on the growth of CRC cells, suggesting its value as a potential anti-tumor candidate for treatment of CRC.关键词
结直肠癌/miRNA-221/增殖/凋亡Key words
colorectal carcinoma/ miRNA-221/ proliferation/ apoptosis分类
医药卫生引用本文复制引用
王伟,孙凯,吴承堂,雷尚通,曾俊杰,伍颖君,李国新..特异性miR-221抑制剂对结直肠癌细胞增殖及凋亡的影响[J].南方医科大学学报,2011,31(4):674-677,4.基金项目
广东省自然科学基金博士启动项目(8451051501000390) （8451051501000390）
广东省医学科研基金(B2010190) （B2010190）
南方医科大学南方医院院长基金(2008C005) （2008C005）
