南方医科大学学报2011，Vol.31Issue(4)：686-689,4.
乙型肝炎病毒聚合酶与UXT蛋白的潜在相互作用鉴定
Identification of ubiquitously expressed transcript as the potential interactor of hepatitis B virus polymerase
摘要
Abstract
Objective To investigate the function of hepatitis B virus polymerase (HBV Pol) in the viral life cycle by screening the proteins interacting with HBV polymerase. Methods The HBV Pol gene was constructed into the pGBKT7 vector. GAL4 yeast two-hybrid system was used to screen the human liver cDNA library to obtain proteins which interacted with HBV Pol. GST-pull down assay was applied to confirm the protein interactions. Results Ubiquitously expressed transcript (UXT) was selected by the yeast two-hybrid system. GST-pull down assay confirmed the in vitro interaction between HBV Pol and UXT. Conclusion UXT is a potential interactor of HBV Pol, and this protein interaction may provide clues of the function of HBV Pol in HBV life cycle.关键词
酵母双杂交/乙肝病毒聚合酶/UXT/蛋白质相互作用Key words
yeast two-hybrid/ HBV polymerase/ ubiquitously expressed transcript/ protein interaction分类
生物科学引用本文复制引用
陈孟璋,邹奕,李君武,李月琴,李弘剑,周天鸿..乙型肝炎病毒聚合酶与UXT蛋白的潜在相互作用鉴定[J].南方医科大学学报,2011,31(4):686-689,4.基金项目
国家科技重大专项课题(2008ZX10002-009) （2008ZX10002-009）
