军医进修学院学报2011，Vol.32Issue(6)：613-615,619,4.DOI:CNKI:11-3275/R.20110323.0845.001
携带SPK1基因的质粒预防术后肠粘连的实验研究
Preventive effect of plasmid-mediated sphingosine kinasel gene transfer on intestinal adhesion after operation
摘要
Abstract
To observe the expression of plasmid sphingosine kinasel (SPK1) in a rat model of intestinal adhesion and the protective effect of SPK1 gene transfer on intestinal adhesion in rats after operation. Methods Mesothelial cells isolated from rats were cultured and transfected with pcDNA3-SPKl. Expression of SPK1 protein was detected by Western blot and activity of SPK1 was assayed by [ γ-32P] ATP incorporation. Transfer efficiency of plasmid vector in mesothelial cells was evaluated using green fluorescent protein (GFP) as a marker. A rat model of cecum brush burn-induced adhesion was established to evaluate the preventive effect of plasmid-mediated SPK1 gene transfer on intestinal adhesion after operation. Results Plasmid pcDNA3-SPKl effectively transfected the damaged mesothelium and mediated the SPK1 gene expression. Plasmid pcDNA3-SPKl gene transfer decreased the intestinal adhesion from 80% to 30% in the rat model of intestinal adhesion after operation. Conclusion Plasmid SPK1 can effectively prevent intestinal adhesion in rats after operation.关键词
肠粘连/鞘氨醇激酶/质粒载体/间皮细胞Key words
Intestinal Adhesion/Sphingosine Kinasel/Plasmid Vector/Mesothelial Cell分类
基础医学引用本文复制引用
郭强,张凯,刘谟焓,李庆芳,孔庆龙..携带SPK1基因的质粒预防术后肠粘连的实验研究 [J].军医进修学院学报,2011,32(6):613-615,619,4.基金项目
国家自然科学基金项目(30901462) （30901462）
解放军总医院科技创新苗圃基金面上项目(09KMM12) （09KMM12）
