军医进修学院学报2011，Vol.32Issue(6)：622-624,3.DOI:CNKI:11-3275/R.20101227.1104.001
IL2、IL15对NK细胞KIR3DL1、NKG2D表达的影响
Effect of IL-2 and IL-15 on expression of KIR3DL1 and NKG2D in natural killer cells
摘要
Abstract
To study the effect of IL-2 and IL-15 on expression of KIR3DL1 and NKG2D in human peripheral blood derived natural killer(NK) cells. Methods NK cells were isolated from PBMNC using mini-magnetic cell-selection(MACS) and NK cell isolation kit(Miltenyi Biotec, Germany), and cultured with stem cell growth medium (SCGM) supplemented with 5% human AB serum in combination with of IL-2 and IL-15 for 15 days. Cytotoxicity to target K562 cells was evaluated, and expression of KIR3DLl(CD158e) and NKG2D in NK cells was analyzed by flow cytometry. Results The cytotoxicity of NK cells was significantly higher after culture than before culture(P<0.01). The expression rate of KIR3DL1 in NK cells was about 10% lower after culture than before culture(P<0.05). No significant difference was found in expression of NKG2D, which was 99% before and after culture. Conclusion The cytotoxicity of NK cells after a long time in vitro culture with IL2 and IL15 significantly increases and down-regulates the expression of KIR3DL1关键词
自然杀伤细胞/白介素2/白介素15/杀伤细胞免疫球蛋白样受体/NKG2DKey words
Natural Killer Cell/Interleukin 2,Interleukin 15/K1P3DL1/NKG2D分类
基础医学引用本文复制引用
李晓红,吴晓雄,达万明,窦立萍,李猛,蔡博,于力,高春记..IL2、IL15对NK细胞KIR3DL1、NKG2D表达的影响[J].军医进修学院学报,2011,32(6):622-624,3.基金项目
国家自然科学基金项目(81070451) （81070451）
