军医进修学院学报2011，Vol.32Issue(6)：625-628,4.DOI:CNKI:11-3275/R.20110309.1538.002
姜黄素对肠缺血再灌注致肝损伤的保护作用
Protective effect of curcumin on liver against intestinal ischemia/reperfusion injury
摘要
Abstract
To study the protective effect of curcumin on liver against intestinal ischemia/reperfusion(I/R) injury. Methods Twenty-one KM mice were randomly divided into sham group, intestinal ischemia group(with 45min ischemia and 24h reperfusion), and curcumin treatment group. A mouse I/R model was established. The mice in intestinal ischemia and curcumin treatment groups were injected with 0.3ml PVP-K30 and 2.5mg/ml curcumin respectively, and repeated them after 6h. The mice were killed after 24h. Pathological changes in intestine and liver were observed with hematoxylin and eosin(HE) staining. Dynamic levels of alarmine transferase(ALT), lactate dehydrogenase(LDH), and nobelium(NO) in liver tissues were measured using biochemical methods. Levels of cytosolic phospholipase A2(cPLA2), phosphorylation of cytosolic phospholipase A2(p-cPLA2), cyclooxygenase-2(COX-2), and β -actin were measured by Western blot. Results The pathological changes in intestine and liver were more significant in curcumin treatment group than in intestinal ischemia group(P<0.01) according to the Chiu' intestinal tissue injury assessment criteria. The ALT level was significantly lower while the NO level was significantly higher in curcumin treatment group than in intestinal ischemia group(P<0.05). No significant difference was found in LDH level between the two groups(P>0.05). Western blot showed that the expression levels of p-cPLA2 and COX-2 in liver were higher in intestinal ischemia group than in curcumin treatment group(P<0.01), and lower in curcumin treatment group than in intestinal ischemia group(P<0.01). No significant difference was found in expression levels of cPLA2 and β -actin between the two groups. Conclusion Curcumin can significantly improve liver damage caused by intestinal I/R by increasing the anti-oxidative ability of liver and regulating the expression of certain inflammation cytokines.关键词
姜黄素/缺血/再灌注损伤/肝脏/胞浆型磷脂酶A2/环氧化酶-2Key words
Curcumin/Ischemia/Reperfusion Injury/Liver/Cytosolic Phospholipase A2/Cyclooxygenase-2分类
基础医学引用本文复制引用
刘晨风,邓子辉,张凯,薛辉,郝秀华,颜光涛..姜黄素对肠缺血再灌注致肝损伤的保护作用[J].军医进修学院学报,2011,32(6):625-628,4.基金项目
国家自然科学基金项目(30670821) （30670821）
国家科技支撑计划项目(2006BAF07B01) （2006BAF07B01）
