南方医科大学学报2011，Vol.31Issue(4)：724-726,3.DOI:CNKI:44-1627/R.20110303.1727.000
单核细胞宫腔灌注联合冻融胚胎移植治疗反复着床失败:附3例报道
Frozen embryo transfer combined with intrauterine administration of autologousperipheral blood mononuclear cells for repeated implantation failure: report of 3 cases
摘要
Abstract
Objective To explore the efficacy of frozen-thawed embryo transfer combined with intrauterine administration of autologous peripheral blood mononuclear cells (PBMCs) in the treatment of repeated implantation failure (RIF). Methods PBMCs obtained from 3 patients with RTF on the day of follicle rupture (natural cycle) or when the endometrial thickness reached 8 mm (hormone replacement cycle) were cultured in the presence of HCG for 48 h. The cultured PBMCs, along with freshly isolated PBMCs, were administered into the uterine cavity of the patterns. Vitrified cleavage-stage embryos or blastocysts transfer was performed on day 3 or 5, respectively. Results Vitrified embryo or blastocyst transfer resulted in pregnancy and healthy live births in all the 3 patients. Conclusion Frozen-thawed embryo transfer combined with intrauterine administration of autologous PBMCs may be an effective and safe approach to the treatment of RIF and may improve the outcomes of assisted reproduction.关键词
反复着床失败/单核细胞,外周血/冻融胚胎移植/体外受精-胚胎移植Key words
repeated implantation failure/ in vitw fertilization and embryo transfer/ frozen-thawed embryo transfer/ peripheral blood mononuclear cells分类
医药卫生引用本文复制引用
陈雷宁,全松,李红,陈思梅,张为青,罗琛,宋亚丽,邢福祺..单核细胞宫腔灌注联合冻融胚胎移植治疗反复着床失败:附3例报道 [J].南方医科大学学报,2011,31(4):724-726,3.基金项目
广东省自然科学基金(9151051501000066) （9151051501000066）
南方医院新技术新项目基金(2006002) （2006002）
